Commonly used consumables for cell culture

1. Vessels related to cell culture

Vessels related to cell culture include: cell culture flasks, cell culture plates, cell culture dishes, erlenmeyer flasks, etc.;

Cell culture vessels must be DNase-free, RNase-free, pyrogen-free, non-toxin certified, sterility level up to 10-6, and materials free from biologically contaminated substances.

In addition to the commonly used cell culture flasks/plates/dishes, there are also some special cell culture containers or tools, such as: Transwell chambers, cell slides, and confocal small dishes. Large-scale cell culture consumables include: multi-layer culture flasks, cell factories, roller bottles, culture bags, etc.

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2. Centrifuge tube (sterile)

According to the shape of the bottom, the centrifuge tubes are divided into flat bottom centrifuge tubes, round bottom centrifuge tubes and pointed bottom centrifuge tubes; according to the closing method of the lid, they are divided into gland centrifuge tubes and screw cap centrifuge tubes. The centrifuge tubes used for cell culture are mainly 15ml and 50ml screw cap conical centrifuge tubes. Centrifuge tubes must be DNase-free, RNase-free, pyrogen-free, non-toxin certified, sterility level up to 10-6, and materials free from biologically contaminated substances.

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3. Serological Pipette (sterile)

In the process of cell culture, it is often necessary to operate a large volume of liquid, and pipettes of various specifications are one of the necessary supplies for the cell culture room.

Serological Disposable plastic pipettes are convenient, safe and accurate. It can meet the requirements of no DNase, no RNase, no pyrogen, no toxin certification, and no biological contamination of the material.

Serological Pipettes are more suitable for large-volume liquid operations that do not require high precision, and belong to constant pipettes.

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4. Cell cryopreservation tube (sterile)

ultra-low temperature preservation tube, a container for low-temperature transportation and storage of cells. The specifications of cryopreservation tubes are 1.5ml, 1.8ml, 2.0ml, and 5.0ml. According to the sample volume and type, you can also choose cryopreservation bags, etc. According to the design of the cap, cryotubes are divided into external rotation cryotubes and internal rotation cryotubes.

For stricter quality control management of the sample library, there are also 2D frozen tubes with QR codes. No matter what kind of cryopreservation container, it must be DNase-free, RNase-free, pyrogen-free, non-toxin certified, sterility level up to 10-6, and materials free of biological contamination and thawed substances.

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5. Disposable cell scraper/spatula

Cell scraper, usually made of high-quality polyethylene material, has relatively good toughness, ensures the protection of cells in the process of collecting cells, and is an auxiliary tool for collecting cells.

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6. Sterilizing filter (sterile)

The pore size is usually 0.22 μm or 0.1 μm for the preparation of medium or related reagents in contact with cells. According to the volume of the sample to be filtered, different filter media can be selected, such as syringe filters, top filters, etc.

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7. Pipette tips(sterile)

It is the most commonly used disposable consumables are used in any application of molecular biology and genetics research. It effectively forms a protective structure between the pipette and the sample to ensure the safety of sampling and dividing. Volumes usually range from 0.1 – 5000ml. Tips used for cell experiments must be DNase-free, RNase-free, pyrogen-free, toxin-free, and materials free from bio-contamination and melted substances.

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8. Other consumables

Cell screens, cryopreservation boxes (for storing and managing cryovials), alcohol lamps, gloves, sterile lab coats, sterile water, liquid storage bottles.

Therefore, when we select consumables for cell culture, the first condition is sterility, especially when cultivating consumables and consumables involving low temperature operations, we must also consider their low temperature tolerance and safety.